But the NFU and Defra have maintained the Government must implement its eradication strategy as soon as possible.
Defra has been considering eight new applications for culling licenses and 21 expressions of interest across Cheshire, Cornwall, Devon, Dorset, Gloucestershire, Herefordshire, Somerset, Wiltshire and Worcestershire.
Natural England’s public consultation, which gathered 939 responses from local people, found more than two-thirds of respondents thought culling could have a negative effect on local businesses, due to people avoiding the area.
Almost 400 raised health and safety concerns around shooting in proximity to pursuits such as walking, camping and wildlife watching.
Claire Bass, executive director of Humane Society International/UK, said the research should serve as a ‘major wake-up call’ to the Government.
She said: “Residents know it is a PR nightmare which will damage local economies including tourism, and many have serious legitimate concerns over the public safety of license-holders running around the countryside in the dark with shotguns, taking pot shots at badgers.
“People do not want this disastrous cull in their backyards and the Government clearly has no public mandate to continue or expand it.”
The NFU stressed bovine TB was endemic across south west England, large parts of the Midlands and beyond.
A union spokesman said: “The fact so many areas have expressed an interest shows how widespread the bTB problem is and how urgently it needs to be dealt with.
“It also shows farmers and landowners support the Government policy and are committed to playing their part.
“The consultation process identified a total of 46 specific material impacts on businesses or people in the nine counties involved.
“We further understand the relevant companies will be notified of the responses which relate to their area and these potential issues will need to be considered and addressed as part of any licence application.”
Defra said it was committed to delivering its 25-year strategy to eradicate the disease and protect the future of UK dairy and beef industries.
The spokesman said: “This includes strengthening cattle testing and movement controls, improving biosecurity on-farm and when trading and badger control in areas where TB is rife.
“This comprehensive approach has worked overseas and is supported by the Government, Defra chief scientists and leading vets.”