The disease kills off bee larva, is highly contagious and difficult to eradicate.
Two cases of the disease were confirmed on Monday (June 13) following laboratory diagnosis by Science and Advice for Scottish Agriculture (SASS).
The AFB infected hived have been destroyed due to there being no permitted treatment for the disease in the UK.
While the disease is devastating for bee keepers, it does not pose any risk to public health and shows no implications for the quality and safety of honey.
The Scottish Government is urging bee farmers and beekeepers to be vigilant for signs of the disease which include sunken cappings on cells, which when uncapped reveal dead larvae in various stages of decomposition.
The larvae have a caramel like, light to dark brown consistency and when drawn out, the decomposing material strings out rather than snapping off.
Bee farmers and beekeepers are asked to notify any suspicion of the disease to Bees_Mailbox@gov.scot.
Beekeepers in the area of this outbreak who are not on BeeBase are requested to register at secure.fera.defra.gov.uk/beebase/public/register.cfm or send their contact details to Bees_Mailbox@gov.scot.